Publications (2016-present)

Papers in peer-reviewed journals (2016 – present)

underline: team members in the Chen lab (supervised/co-supervised); #: co-first authors; *: corresponding author

  1. Hong J#, Rosental L#, Xu Y#, … Chen G*, Brotman Y*, Zhang D*, Shi J*. Genetic architecture of seed glycerolipids in Asian cultivated rice. Plant, Cell & Environment, 2022, accepted.
  2. Wang J, Singer SD, Souto B, Asomaning J, Ullah A, Bressler D, Chen G*. Current progress in lipid-based biofuels: Feedstocks and production technologies. Bioresource Technology, 2022, 351:127020. [link]
  3. Zhou Q#, Jayawardhane K#, Strelkov SE, Hwang SF, Chen G*. Identification of Arabidopsis phospholipase A knockout mutants with increased susceptibility to Plasmodiophora brassicaeFrontiers in Plant Science, 2022, 13:799142.
  4. Singer SD*, Hughes, KB, Subedi U, Dhariwal GK, Kader K, Acharya S, Chen G, Hannoufa A. The CRISPR/Cas9-mediated modulation of SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE 8 (SPL8) in alfalfa leads to distinct phenotypic outcomes. Frontiers in Plant Science, 2022, 12:774146.
  5. Shan Q, Tian G, Han Z, Hui H, Yamamoto M, Hao M, Wang J, Wang K, Sang X, Qin L, Chen G*, Cao G*. Toxicity of Tetradium ruticarpum: Subacute toxicity assessment and metabolomic identification of relevant biomarkers. Frontiers in Pharmacology, 2022, 13:803855.
  6. Xu Y#, Pan X#, Lu J, Wang J, Shan Q, Stout J, Chen G*. Evolutionary and biochemical characterization of a Chromochloris zofingiensis MBOAT with wax synthase and DGAT activity. Journal of Experimental Botany, 2021, 72: 5584-5598. [Link]
  7. Xu YCaldo KMP, Singer SD, Miekiewska E, Greer MS, Tian B, Dyer JM, Smith M, Zhou X-R, Qiu X, Weselake RJ, Chen G*Physaria fendleri and Ricinus communis LCAT-like phospholipases selectively cleave hydroxy acyl chains from phosphatidylcholine. Plant Journal, 2021, 105(1), 182-196. [link]
  8. Yu L, Liu D, Wei L, Yu P, Wang J, Hu Z, Zhang Y, Zhang S, Yang Z, Chen G, Yao X, Yang Y, Zhou Y, Wang X, Lu S, Dai C, Yang Q, Guo L*. BnTIR: an online transcriptome platform for exploring RNA-seq libraries for oil crop Brassica napusPlant Biotechnology Journal, 2021, 19 (10): 1895-1897.
  9. Cui Y, Zeng X, Xiong Q, Wei D, Liao J, Xu YChen, G, Zhou Y, Dong H, Wang H, Liu Z, LI J, Guo L, Jung C, He Y*, Qian W*. Combining QTL and co-expression analysis allowed identification of new candidates for oil accumulation in rapeseed. Journal of Experimental Botany, 2021, 72(5), 1649-1660 [link].
  10. Wang JXu Y, Holic R, Yu X, Singer SD, Chen G*. Improving the production of punicic acid in baker’s yeast by engineering genes in acyl channeling processes and adjusting precursor supply. Journal of Agricultural and Food Chemistry, 2021, 69: 9616-9624 [link].
  11. Singer SD*, Jayawardhane K, Jiao C, Weselake RJ, Chen G*. The effect of AINTEGUMENTA-LIKE 7 over-expression on seed fatty acid biosynthesis, storage oil accumulation and the transcriptome in Arabidopsis thaliana. Plant Cell Reports, 2021, 40 (9): 1647-1663. [Link]
  12. Singer SD*, Subedi U, Lehmann M, Hughes KB, Feyissa BA, Hannoufa A, Shan B, Chen G, Kader K, Polo RO, Schwinghamer T, Dhariwal GK, Acharya S. Identification of differential drought response mechanisms in Medicago sativa subsp. sativa and falcata through comparative assessments at the
    physiological, biochemical, and transcriptional levels. Plants, 2021, 10 (10): 2107.
  13. Xin H, Ma T, Xu YChen G, Chen Y, Villot C, Steele MA, Guan LL*. Characterization of fecal branched-chain fatty acids profiles and their associations with fecal microbiota in diarrheic and healthy dairy calves, Journal of Dairy Science, 2021, 104(2), 2290-2301. [link]
  14. Falarz L, Xu Y, Caldo KMP, Garroway CJ, Singer SD, Chen G*. Characterization of the diversification of phospholipid:diacylglycerol acyltransferases in the green lineage. Plant Journal, 2020, 103: 2025-2038 [link]
  15. Xu Y, Mietkiewska E, Shah S, Weselake RJ, Chen G*. Punicic acid production in Brassica napusMetabolic Engineering, 2020, 62, 20-29. [Link]
  16. Xu Y, Caldo KMP, Falarz L, Jayawardhane K, Chen G*. Kinetic improvement of an algal diacylglycerol acyltransferase 1 via fusion with an acyl-CoA binding protein, Plant Journal, 2020, 102, 856-871. # featured article of the issue [link]
  17. Subedi U, Ozga J, Chen G, Foroud NA, Singer SD*. CRISPR/Cas-mediated genome editing for the improvement of oilseed crop productivity. Critical Reviews in Plant Sciences, 2020, 39, 195-221. [link]
  18. Nguyen T, Xu Y, Abdel-Hameed M, Sorensen JL, Singer SD, Chen G*. Characterization of a type-2 diacylglycerol acyltransferase from Haematococcus pluvialis reveals possible allostery of the recombinant enzyme, Lipids, 2020, 55: 425-433
  19. Xu Y, Singer SD, Chen G. Improving oil production in plants and microalgae by engineering performance-enhanced diacylglycerol acyltransferase 1, Inform2020, 31, 20-23. [link]
  20. Lu J, Xu Y, Wang J, Singer SD*, Chen G*. The role of triacylglycerol in plant stress response, Plants2020, 9, 472
  21. Shan Q, Tian G, Wang J, Hui H, Shou Q, Fu H, Hao M, Wang K, Wu X, Cao G*, Chen G, Qin L*. Change in the active component of processed Tetradium ruticarpum extracts leads to improvement in efficacy and toxicity attenuation. Journal of Ethnopharmacology, 2020, 264, 113292
  22. Tian B*, Sun M, Jayawardana K, Wu D, Chen G. Characterization of a PLDζ2 homology gene from developing castor bean endosperm. Lipids2020, 55: 537-548
  23. Singer SD*, Chatterton S, Soolanayakanahally RY, Subedi UChen G, Acharya SN. Potential effects of a high CO2 future on leguminous species, Plant-Environment Interactions, 2020, 1:67-94 [link]
  24. Jayawardhane K, Singer SD, Ozga JA, Rizvi SM, Weselake RJ, Chen G*. Seed-specific down-regulation of Arabidopsis CELLULOSE SYNTHASE 1 or 9 reduces seed cellulose content and differentially affects carbon partitioning, Plant Cell Reports, 2020 39(7), 953-969
  25. Wijekoon CP, Singer SD, Weselake RJ, Petri JR, Chen G, Singh S, Eastmond PJ, Acharya S*. Down-regulation of key genes involved in carbon metabolism in Medicago truncatula results in increased lipid accumulation in vegetative tissue. Crop Science, 2020, 60: 1798-1808
  26. Subedi U, Jayawardhane K, Pan X, Ozga J, Chen G, Foroud NA, Singer SD*. The potential of genome editing for improving seed oil content and fatty acid composition in oilseed crops. Lipids, 2020, 55: 495-512
  27. Patel D, Goruk S, Newell M, Chen G, Richard C, Field CJ*. Feeding a bioactive oil enriched in stearidonic acid during early life influences immune system maturation in neonatal Sprague-Dawley rats, The Journal of Nutrition, 2020, 150 (3): 606-615
  28. Xin H, Xu Y, Chen Y, Chen G, Steele MA, Guan LL*. Odd-chain and branched-chain fatty acids concentrations in bovine colostrum and transition milk and their stability under heating and freezing treatments, Journal of Dairy Science, 2020, 103(12): 11483-11489 [link]
  29. Wijekoon CP, Singer SD, Weselake RJ, Petri JR, Singh S, Jayawardhane K, Shah S, Chen G, Eastmond PJ, Acharya S*. Enhancement of total lipid production in vegetative tissues of alfalfa and sainfoin using chemical mutagenesis, Crop Science, 2020, 60, 2990-3003 [link]
  30. Caldo KMP#, Xu Y#, Falarz L#, Jayawardhane K, Acedo JZ, Chen G*. Arabidopsis CTP:phosphocholine cytidylyltransferase 1 is phosphorylated and inhibited by sucrose nonfermenting 1–related protein kinase 1 (SnRK1). Journal of Biological Chemistry2019, 294, 15862-15874. #:co-first authors. recommended in F1000Prime as being of special significance in its field [link]
  31. Tian B*, Lu T, Xu Y, Wang R, Chen G*. Identification of genes associated with ricinoleic acid accumulation in Hiptage benghalensis via transcriptome analysis. Biotechnology for Biofuels, 2019, 12:16.
  32. Xu Y, Falarz LChen G*. Characterization of type-2 diacylglycerol acyltransferases in the green microalga Chromochloris zofingiensisJournal of Agricultural and Food Chemistry, 2019, 67 (1), 291–298.
  33. Falarz L, Xu Y, Singer SD, Chen G*. A fluorescence-based assay for quantitative analysis of phospholipid:diacylglycerol acyltransferase activity. Lipids, 2019, 54(9): 571-579.
  34. Xu Y, Caldo KMP, Jayawardhane K, Ozga J, Weselake RJ, Chen G*. Transferase interactome that may facilitate channeling of polyunsaturated fatty acid moieties from phosphatidylcholine to triacylglycerol. Journal of Biological Chemistry2019, 294, 14838-14844. Accelerated Communications; Featured article of the Oct 11, 2019 issue [link]
  35. Lin Y, Chen G*, Mietiewska E, Song Z, Caldo KMP, Singer SD, Dyer J, Smith M, McKeon T, Weselake RJ*. Castor patatin-like phospholipase a IIIβ facilitates removal of hydroxy fatty acids from phosphatidylcholine in transgenic Arabidopsis seeds, Plant Molecular Biology, 2019, 101, 521-536. Featured article of the Dec, 2019 issue [link]
  36. Tunsagool P, Wang X, Leelasuphakul W, Jutidamrongphan W*, Phaonakrop N, Jaresitthikunchai J, Roytrakul S, Chen G, Li L*. Metabolomic study of stress responses leading to plant resistance in mandarin fruit mediated by preventive applications of Bacillus subtilis cyclic lipopeptides. Postharvest Biology and Technology, 2019, 156: 110946
  37. Xu Y, Caldo K, Holic R, Mietkiewska E, Ozga J, Rizvi SM, Chen G, Weselake RJ*. Engineering Arabidopsis long-chain acyl-CoA synthetase 9 variants with enhanced enzyme activity. Biochemical Journal, 2019, 476: 151-164.
  38. Caldo K, Shen W, Xu Y, Hanley-Bowdoin L, Chen G, Weselake R*, Lemieux MJ*. Diacylglycerol acyltransferase 1 is activated by phosphatidate and inhibited by SnRK 1‐catalyzed phosphorylation. Plant Journal2018, 96 (2): 287-299.
  39. Xu Y, Caldo K, Pal-Nath D, Ozga J, Lemieux MJ, Weselake RJ, Chen G*. Properties and biotechnological applications of acyl-CoA:diacylglycerol acyltransferase and phospholipid:diacylglycerol acyltransferase from terrestrial plants and microalgae. Lipids, 2018, 53 (7): 663-688. #Among the journal’s top downloaded recent papers in 2017-2018.
  40. Jayawardhane K, Singer SD, Weselake RJ, Chen G*. Plant sn-glycerol-3-phosphate acyltransferases: biocatalysts involved in the biosynthesis of intracellular and extracellular lipids. Lipids, 2018, 53:469-480. #Among the journal’s top downloaded recent papers in 2017-2018.
  41. Holic R*, Xu Y, Caldo K, Singer SD, Field CJ, Weselake RJ, Chen G*. Bioactivity and biotechnological production of punicic acid. Applied Microbiology and Biotechnology, 2018, 102: 3537-3549.
  42. Xu Y, Holic R, Li D, Pan X, Mietkiewska E, Chen G, Ozga J, Weselake RJ*. Substrate preferences of long-chain acyl-CoA synthetase and diacylglycerol acyltransferase contribute to the enrichment of flax seed oil with α-linolenic acid. Biochemical Journal, 2018, 475: 1473-1489.
  43. Xu Y, Chen G, Greer M, Caldo K, Ramakrishnan G, Shah S, Wu L, Lemieux MJ, Ozga J, Weselake RJ. Multiple mechanisms contribute to increased neutral lipid accumulation in yeast producing recombinant variants of plant diacylglycerol acyltransferase 1. Journal of Biological Chemistry, 2017, 292:17819-17831.
  44. Chen G *, Xu Y, Rodrigo S, Clado K, Vanhercke T, EI Tahchy A, Niesner N, Chen Y, Mietkiewska E, Weselake RJ*. High-performance variants of plant diacylglycerol acyltransferase 1 generated by directed evolution provide insights into structure function. Plant Journal, 2017, 92: 167-177.  # featured article of the issue [link-1] & the subject of a Research Highlight article [link-2]
  45. Singer S, Chen G (Co-first author), Mietkiewska E, Tomasi P, Jayawardhane K, Dyer J, Weselake RJ*. Arabidopsis GPAT9 contributes to synthesis of intracellular glycerolipids but not surface lipids. Journal of Experimental Botany, 2016, 67: 4627-4638.

Book

  • Chen G, Weselake RJ, Singer SD (eds), Plant Bioproducts, Springer, 2018 (link)

Book chapters

  • McDonald K, Singer SD, Chen G. Recent advances in the biosynthesis and metabolic engineering of storage lipids and proteins in seeds. In: Bandara N and Ullah A (eds.) Functional Materials from Lipids and Proteins. Royal Society of Chemistry, 2022. Accepted.
  • Subedi U, Hughes KB, Chen G, Hannoufa A, Singer SD. Eliciting targeted mutations in Medicago sativa using CRISPR/Cas9-mediated genome editing: a potential tool for the improvement of disease resistance. In: Plant-Pathogen Interactions, Methods in Molecular Biology series, Springer Nature, 2021. Accepted.
  • Xu Y, Jayawardhane K, Singer SD, Chen G*. Methods for assessing triacylglycerol biosynthesis and the activities of diacylglycerol acyltransferase and phospholipid: diacylglycerol acyltransferase in plant systems. In: Lipid-mediated Signaling Transduction (2nd edition), Murphy EJ, Rosenberger T, Golovko M, eds. CRC Press: 2019, accepted.
  • Eskin NAM*, Aladedunye F, Unger EH, Chen G, Shah S, Jones PJ. Canola oil. In Bailey’s Industrial Oil and Fat Products (7th Edition), Shahidi F. Ed. Wiley: 2020, vol 2, pp 57-120.
  • Weselake RJ*, Singer SD, Chen G, An integrated approach to plant bioproduct production. In Plant Bioproducts, Chen G, Weselake RJ, Singer SD, Eds. Springer: 2018; pp 27-40.
  • Weselake RJ*, Singer SD, Chen G, Introduction to plant biomolecules and cellular metabolism. In Plant Bioproducts, Chen G, Weselake RJ, Singer SD, Eds. Springer: 2018; pp 9-25.
  • Weselake RJ*, Chen G, Singer SD, Building a case for plant bioproducts. In Plant Bioproducts, Chen G, Weselake RJ, Singer SD, Eds. Springer: 2018; pp 1-8.
  • Falarz LJ, Singer SD, Chen G*, Biomass-derived building block chemicals. In Plant Bioproducts, Chen G, Weselake RJ, Singer SD, Eds. Springer: 2018; pp 177-200.
  • Falarz LJ, Deyholos MK, Chen G*, Plant carbohydrates and production of renewable biofuel from starch, sugar, and cellulose. In Plant Bioproducts, Chen G, Weselake RJ, Singer SD, Eds. Springer: 2018; pp 87-107.
  • Falarz LJ, Deyholos MK, Chen G*, Materials and related bioproducts from plant carbohydrates. In Plant Bioproducts, Chen G, Weselake RJ, Singer SD, Eds. Springer: 2018; pp 109-120.
  • Harwood JL*, Woodfield HK, Chen G, Weselake RJ*, Modification of oil crops to produce fatty acids for industrial applications. In Ahmad MU (eds), Fatty Acids: Chemistry, Synthesis, and Applications, Academic Press and AOCS Press, San Diego, 2017
  • Chen G*. Chapter 11.4 Emerging industrial oilseed crops: Jatropha. In McKeon TA, Hildebrand DF, Weselake RJ, Hayes DG, (eds), Industrial Oilseed Crops, Academic Press and AOCS Press, San Diego, 2016
  • Chen G*. Chapter 11.6 Emerging industrial oilseed crops: Physaria. In McKeon TA, Hildebrand DF, Weselake RJ, Hayes DG, (eds), Industrial Oilseed Crops, Academic Press and AOCS Press, San Diego, 2016
  • Chen G*. Chapter 11.8 Emerging industrial oilseed crops: Pennycress. In McKeon TA, Hildebrand DF, Weselake RJ, Hayes DG, (eds), Industrial Oilseed Crops, Academic Press and AOCS Press, San Diego, 2016

Special Issues

  • Murphy EJ*, Chen G. Contributions Honoring the Career of Randall J. Weselake: From Plant Lipid to Cancer Lipid Biochemistry, Lipids, 2020, 55 (5)
  • Kourist R, Chen G. Recent trends in biotechnology in South and South East Asia. Biocatalysis and Agricultural Biotechnology, 2019

Publications before 2016 – see the subpage